Fertility NEWS LETTER
Ideal fertility : ICSI / IVF & Genetic Center India
Vol VIII,issue 5, May 2010
In this issue
- Thalassemia screening by Dr.Anupama Solonki
- Paternal Contribution of aneuploidy in Assisted reproduction technology
- Summer course in Biotechnology for University students of Biotech
In previous issue
- Clomiphene citrate : some useful points
- GnRh antagonist regimen in IUI and IVF
- Fellowship course in Reproductive endocrinology and Infertility
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Dear Colleges
Hello
In this issue we are putting two topics ,one on thalassemia and the other one is the effect of paternal age on fetal defects.
One of our colleague Dr.Anpama Solonki has contributed one topic on thalassemia screening, I am thankful to her for her co-operation to improve the content of this newsletter.
Anemia in pregnancy is very common and we found that many of the women are carriers of various hemoglobiopathies. Proper screening and counseling will definitely reduce the burden of these disorders.
Our aim in this institution is to search for the reasons of anemia from macro to molecular level. Correct diagnosis will help to reduce the anemia and in-turn will reduce the maternal mortality and morbidity.
Other topic is ,how father is responsible for chromosomal defects in fetuses conceived by assisted reproductive technologies.
We are conducting or 10th annual summer course in biotechnology for biotech students who are pursuing their degree courses in various universities.
Hope you will enjoy the topics
With best wishes
Dr. D’Pankar Banerji
1.THALASSEMIA SCREENING
Hemoglobin A ( alfa2/beta2) is the major hemoglobin found in adult and children.
Hb A2 ( alfa2/delta2) and HbF ( alfa2/gamma2) and their concentrations are 2-2.3% and 0.2-1.0% in adult life respectively.
Alfa chains synthesis is directed by two alfa genes, alfa1 and alfa2, on chromosome 16 ,and beta and delta chains synthesis by single genes beta and delta on chromosome 11. Gamma chain synthesis is directed by two genes, G-gamma and A-gamma also on chromosome 11.
METHODS FOR INVESTIGATION OF SUSPECTED THALASSEMIA
- Full blood count with red cell indices
- HbA2 measurement
- Automated high performance liquid chromatography
- Quantitation of Hb F
- Assessment of iron status
- Demonstration of red cell inclusion bodies
- DNA analysis
2. Paternal Contribution of aneuploidy in Assisted reproduction technology
Numerical and structural abnormalities in sperm increase somewhat with aging, but since the increase starts over forty and is initially very low, sperm aneuploidies are not clinically relevant in the fertile male population. However , gonosomic aneuploidy and trisomy 21 have been demonstrated to have a possible paternal origin.
Among the infertile population entering ART treatment , a male factor is frequently involved . almost 30-40 percent of men suffer of some degree of OAT (oligo-astheno-terato-zoospermia) and 5-8 percent need to use sperm extracted from the testis because of nonobstructive azoospermia (TESE) or from epididymis because of obstructive azoospermia (MESA).
Studies applying FISH ( fluorescent in-siu hybridization) in human sperm start to show a higher frequency of aneuploidy in OAT patients and in TESE samples compared to normospermic ,but still some concerns are present regarding the reliability of the test and its clinical relevance.
In one study from Italy shows that very few (<2 percent) normospermic men had semen samples with a high percentage of abnormal chromosomal complement , while the same figure ranged from 12 percent (moderate OAT) to 89 percent (testicular samples) in the infertile male population. The mean percentage of aneuploid spermatozoon was 1.27 % in normospermic samples, 4.02 % in severe OAT, and 13.7 % in TESE, clearly showing that testicular sperms are significantly more prone to aneuploidy than ejaculated sperms.
Severe OAT and TESE spermatozoa (non-obstructive azoo) generate significantly higher incidence of aneuploid embryos compared to other groups, strongly suggesting a paternal contributions to embryonic aneuploidies when severe infertile samples are used for ICSI .
3. SUMMER COURSE PROGRAM
n Medical Biotechnology
7th June 2010 - 17th June2010
Time 8.00am-1.00pm
Program :
Day 1
Theory :
- Introduction to cell biology
- Microscopy
Practical :
Microscopy :Stereozoom, Trinocular photomicrography, Inverted Microscopy
Day 2
Theory
- Cell cycle and cell division
- Primary tissue culture, introduction to tissue culture
Practical :
Culture media preparation for tissue culture
Day 3
Theory
- Detection and measurement of genetic variation
- Monohybrid/dihybrid, mutations, Mendelian genetics
Practical :
Planting
Day 4
Theory :
- Replication
- ranscription and translation
Practical :
DNA isolation from blood
Day5
Theory
- ovulation induction for in vitro fertilization
- Human ,genetic diseases
Practical :
PCR demonstration
Day6
Theory :
Embryo biotechnology and stem cells
Practical:
- Hands on retrieval of mammalian eggs under stereomicroscope
- in-vitro maturation of oocyte
Day7
Practical:
Harvesting of culture
In-vitro maturation of oocytes
Day8
Theory :
Single Gene disorders
Practical:
Cryopreservation of Spermatozoa and oocytes
Day9
Theory :
Immunogenetics,basic
Practical:
Banding / slide preparation /identification of chromosomes harvested earlier
Day10
Seminar by the participants
Certificate distribution
Faculty :
1. Dr.D'Pankar Banerji, Gynecologist and Infertility specialist
2. Dr. Mrs. Rinku Banerji ,Pathologist and Embryologist
Fees : Rs.5000.00
Phone Number to contact : 2627711
Stay can be arranged at nearby hotels at a reasonable rates at an extra cost
Archives |
- Vol VIII, Issue 11, Nov 2010
- Vol VIII, issue 6, June,2010
- Vol VIII,issue 5, May 2010
- Vol VIII,issue 4, April 2010
- Vol VIII Issue 3, March 2010
- Vol VIII, Issue 1,Jan 2010
- Vol VII, Issue 12,Dec.2009
- Vol VII, Issue 11,Nov.2009
- Vol VII, Issue 10,Oct.2009
- Vol VII, Issue 9, Sep.2009
- Vol VII, Issue 8, Aug 2009
- Vol VII, Issue 7,July 2009
- Vol VII, Issue 6,June 2009
- Vol VII Issue 4 april 2009
- Vol VI, Issue 9, Sep 2008
- Vol Vi Issue 8, aug 2008
- Vol Vi Issue 7, july 2008
- Vol VI, Issue 6, June 2008
- Vol V, Issue 17, may 2008
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- Vol IV, Issue 16, April 2008
- Vol III, Issue 15, March 2008
- Vol I & II, Issue 13-14, Jan Feb 2008
- Vol IV, Issue 12, December 2007
- Vol IV, Issue 11, November 2007
- Vol IV, Issue 10, October 2007
- Vol IV, Issue 9, September 2007
- Vol IV, Issue 8, August 2007
- Vol IV, Issue 7, July 2007
- Vol IV, Issue 6, June 2007
- Vol IV, Issue 5, May 2007
- Vol IV, Issue 4, April 2007
- Vol IV, Issue 3, March 2007
- Vol IV, Issue 2, FEB_2007
- Vol IV, Issue1, Jan 2007
- Vol III, Issue 9, Nov Dec 2006
- Vol II, issue7, July 2005
- Vol II, Issue4 April 2005
- Vol II, Issue3, March 2005
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