Fertility NEWS LETTER

Ideal fertility : ICSI / IVF & Genetic Center India

Vol II, Issue 4 April 2005

In This issue

  1. Premature ovarian failure
  2. Summer course on Biotechnology

Dear Colleges
Hello

Hope holy was full of joy. We are conducting one CME workshop on ovulation induction and sperm preparation on 3rd April 2005. Our next summer course on biotechnology will be starting from 17th may to 27th may 2005.

In this issue we are discussing one small topic of premature ovarian failure ,it really baffles sometimes and the lady goes into great dilemma regarding the further treatment.

With best regards,

Sincerely yours,
Dr. D’Pankar Banerji

1.Premature Ovarian Failure ( POF )

Diagnostic Criteria
  1. Amenorrhea lasting more than 4 months
  2. Age less than 40 yrs, and
  3. Serum Follicular stimulating hormone > 40 mIU/ml on two occasion

But there are reports that ,females with secondary amenorrhea may have intermittent ovarian function in 24% cases and pregnancy in 8% of cases.
Etiology of POF

Ovarian follicle depletion

Deficient initial follicle number
Accelerated follicular atresia
Autoimmunity

Ovarian follicular dysfunction

Enzyme deficiency
Autoimmunity
Lymphocytic oophoritis
Signal defects
Gene mutation
Iatrogenic
Idiopathic
Several autoimmune disorders have been associated with POF ;most common is hypothyroidism with incidence of 27 % ,followed by diabetes mellitus (2.5 % ) and Addison’s disease  ( 2.5 % )

Karyotype should be done in all cases if there is POF before 30 yrs. Y chromosome in any cell line necessitated gonadectomy to prevent gonadoblastoma

2. Summer course for biotechnology students

6th batch
Date : 17-27th May. 2005

DAY 1

THEORY :

  1. Introduction to cell biology
  2. Microscopy

PRACTICAL:
Microscopy: Stereo zoom , Trinocular, photomicrography ,Inverted Microscopy

DAY 2

THEORY

  1. Cell cycle and cell division
  2. Primary tissue culture, introduction to tissue culture

PRACTICAL:
Culture media preparation for tissue culture

DAY 3

THEORY

  1. Detection and measurement of genetic variation
  2. Monohybrid/dihybrid, mutations, Mendelian genetics

PRACTICAL
Planting

DAY 4

THEORY

  1. Replication
  2. Transcription and translation

PRACTICAL
DNA isolation from blood

DAY 5

THEORY

  1. Ovulation induction for in vitro fertilization
  2. Human , genetic diseases

PRACTICAL
PCR : hands on

DAY 6

THEORY

  1. Embryo biotechnology and stem cells

PRACTICAL
1.Hands on retrieval of mammalian eggs under stereomicroscope
2.in-vitro maturation of oocyte

DAY 7

PRACTICAL
Harvesting of culture
In-vitro maturation of oocytes

DAY 8

THEORY
Single Gene disorders

PRACTICAL
Cryopreservation of Spermatozoa and oocytes

DAY 9

THEORY
Immunogenetics,basic

PRACTICAL
Banding / slide preparation /identification of chromosomes harvested earlier

DAY 10

Seminar by the participants
Certificate distribution

10 STUDENTS
Course Fee : Rs.8,000

Course fee includes Lunch
(Stay is not included )

FACULTY : DR.D’PANKAR BANERJI, Gynecologist and Infertility specialist
DR.MRS.RINKU BANERJI, Pathologist and Embryologist

B. Sc.and M.Sc.biotech and biosciences are preferred

Ready made culture media for IUI, freshly prepared on demand, is available, Contact for details at, Ideal Fertility : IVF and Genetic center

We are now started working as Satellite center for IVF and ICSI for few nearby centers in Mahakaushal region of MP (you stimulate the patients we will carryout fertilization and embryo transfer and patient is referred back): for details contact, Ideal Fertility: IVF and Genetic center

Facilities Available at Ideal Fertility : IVF and Genetic Center

1.IVF and ICSI
2. Chromosomal Analysis and Karyotyping
3.Prenatal diagnosis of Beta thalassemia and other genetic disease ( by Chorion biopsy and Amniocentesis)
4. Endoscopic Surgery .Laparoscopic and Hysteroscopic
5.Nonsurgical clearing of fallopian tube
6.Interventional and conventional sonography
7.Embryo and sperm cryofreezing
8.DNA PCR for Tuberculosis

Team At Ideal Fertility : IVF and Genetic Centre

1.Dr.D’Pankar Banerji ,Gynecologist and Infertility specialist
2.Dr.Mrs.Rinku Banerji, Embryologist
3.Ms.Usha and Shushma : Nurses

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